1.
Which of the following regarding flavin adenine dinucleotide is untrue?
A)
It can participate in one electron reactions
B)
The reactive portion is an isoalloxazine ring
C)
The oxidized form can accept two electrons and two protons
D)
It is often used in the conversion of alkanes to alkenes
E)
The reduced form is FAD

2.
The conversion of pyruvate to lactate requires:
A)
FAD
B)
FADH2
C)
NADH
D)
NAD+
E)
ATP

3.
Which statement about acetyl CoA is untrue?
A)
the acetyl group is linked to CoA by a thioester bond
B)
the free energy of hydrolysis is approximately -7.5 kcal/mole
C)
it is formed via a reaction catalyzed by the pyruvate dehydrogenase complex
D)
acetyl CoA carries an activated acetyl group
E)
acetyl CoA is a carrier of three carbon fragments

4.
The protein which catalyzes the reduction of O2 to H2O in oxidative phosphorylation is:
A)
cytochrome c
B)
an iron-sulfur cluster
C)
cytochrome reductase
D)
Q-cytochrome c oxidoreductase
E)
cytochrome oxidase

5.
The substrate for aconitase is:
A)
succinyl-CoA
B)
citrate
C)
oxalosuccinate
D)
α-ketoglutarate
E)
oxaloacetate

6.
Figure 16-4 shows a reaction. This is catalyzed by:

A) aldolase B) triose phosphate isomerase
C)
glyceraldehyde 3-phosphate dehydrogenase
D)
phosphofructokinase
E)
phosphoglycerate mutase

7.
Consider the reaction A 6 B + C. This reaction has a ΔGo’ value of +3 kcal/mole. It is coupled to a reaction B 6 D with ΔGo’ value of -6 kcal/mole. The percentage of C in the A + C reaction mixture at equilibrium is closest to:
A)
99%
B)
90%
C)
50%
D)
10%
E)
1%

8.
Compounds with high phosphate transfer potential include
A)
phosphoenol pyruvate, acetyl phosphate, and AMP
B)
acetyl phosphate, creatine phosphate and glucose 1-phosphate
C)
phosphoenol pyruvate, acetyl phosphate, and glucose 1-phosphate
D)
acetyl phosphate, phosphoenol pyruvate, and creatine phosphate
E)
creatine phosphate, phosphoenol pyruvate, and glucose 6-phosphate

9.
Loss of CO2 will occur most readily from a:
A)
α-ketoacid
B)
b-ketoacid
C)
β-ketoalcohol (β-hydroxyketone)
D)
1,2-diketone
E)
1,3-diketone

10.
In which step of the citric acid cycle is FADH2 formed?
A)
the conversion of fumarate to malate
B)
the conversion of malate to oxaloacetate
C)
the conversion of succinate to fumarate
D)
the conversion of citrate to isocitrate
E)
the conversion of oxaloacetate to citrate

11.
Figure 17-1. Shown is a figure of:

A) cis-aconitate B) citrate C) isocitrate D) oxalosuccinate E) a-ketoglutarate 12.
Figure 17-2. We did not have time this semester to study the biosynthesis of sedoheptulose. From your knowledge of biochemistry you can say that this is most likely formed by (consider the immediate preceding step):

A) a reaction which uses the co-factor thiamine pyrophosphate
B)
a reaction which uses the co-factor CoASH
C)
aldol condensation of a 3-carbon fragment with a 4-carbon fragment
D)
loss of CO2 from an 8-carbon fragment in which the CO2 was attached to the carbon indicated by the arrow
E)
loss of water from a 7-carbon fragment

13.
Figure 16-3. The structure of isopropylmalate is shown on the last page. From your knowledge of biochemistry, you can say that this is most likely to:

A) be reduced by NADH
B)
be reduced by FADH2
C)
react with TPP
D)
lose CO2
E)
lose water

14.
Figure Amanitin. Amanitin inhibits 5S RNA by 50% at approximately (ng = nanogram; mg = milligram):

A)
1 ng/ml
B)
10 ng/ml
C)
100 ng/ml
D)
0.01 mg/ml
E)
1 mg/ml

15.
Which statement about succinate-Q reductase is untrue?
A)
FADH2 is formed in the citric acid cycle in the oxidation of succinate to fumarate by succinate dehydrogenase
B)
succinate dehydrogenase is part of the succinate-Q reductase complex (Complex II)
C)
the succinate-Q reductase complex is an integral membrane protein of the inner mitochondrial membrane
D)
the electrons from FADH2 are transferred to Fe-S centers and then to ubiquinone for entry into the electron transport chain
E)
FADH2 leaves the complex after