individual was able to determine the total amount of protein in the unknown substance. The steps I followed are listed below: 1. Set the wavelength on the spectrophometer at 540nm. Allow the spec to warm up for at least 15 minutes. 2. Label 9 test tubes/cuvettes – one blank, two cuvettes for each S1, S2, S3, and U. 3. Into each tube, pipette 1ml of 0.9% NaCl using the 10ml volumetric pipette. 4. Using the semi-automated (Eppendorf) pipette, add 20ul of standard or unknown to the appropriate…
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