btained by multiplying these two. Five repeated measurements were performed for each sample and their average was taken.
3.2.5.3 FTIR- Spectroscopy
The IR spectra of starches were run as KBr pellets on FT-IR spectrometer at Central Instrumental Laboratory, Guru Jambheshwar University of Science and Technology, Hisar, Haryana, India.
3.2.5.4 Microscopic examination of corn starch
Reagents
Iodine- Potassium iodide solution (I2-KI Sol): Dissolve 2 g of KI and 0.2 g iodine in 100 ml of water.
Starch was microscopically examined by the method of Johansen (1940).
Procedure
A small amount of starch was stained in iodine -potassium iodide solution for 10 min. Washed in water thoroughly and examined using a microscope. Staining of starch granules resulted …show more content…
Procedure
A suspension of starch (5 g, dwt) in 75 ml distilled water was agitated for 1 hour and centrifuged (3000 g) for 10 min. The free water was removed from the wet starch, which was then drained for 10 min. The wet starch was then weighed. Wt. of residual starch (g) x 100
Water binding capacity (%) = __________________________ Wt. of sample (g)
3.2.6.2 Oil absorption capacity (OAC)
OAC of the starches was determined using the method described by Yamazaki (1953), as modified by Medcalf and Gilles (1965).
Procedure
A suspension of starch (5 g, dwt) in 75 ml distilled water was agitated for 1 hour and centrifuged (3000 g) for 10 min. The free water was removed from the wet starch, which was then drained for 10 min. The wet starch was then