Table of Contents
Topic Question 2
Hypothesis 2
Materials 2
Safety Precautions 2
Procedures 3
Data Analysis 3
Hazard levels: biological 4
Disposal Methods 4
Bibliography (Citation) 4

Topic Question
How does hydrochloric acid affect Spirogyra, Volvox, and Micrasteria Cellular Structure?
Hypothesis
Spirogyra will be most affected by the acid rain because its chloroplasts are ribbon shaped and spirally arranged giving the hydrochloric acid more access to it while Volvox, and Micrasteria won’t be as sensitive to the hydrochloric acid.
Materials
Algae: Spirogyra, Volvox, and Micrasteria
Hydrochloric acid
3 test tubes
A microscope to observe the algae’s cellular structure as affected by the acid rain
A gridded microscopic slide
Safety Precautions
Before we begin our experiment, we will need to prepare for it. To begin we need to make sure that our workstation is clean and tidy. Then put on our goggles so that we can protect our eyes. Aprons also need to be worn to protect our clothes because certain substances could stain or burn them. Furthermore Gloves should be worn, to protect our hands, so that they won’t come in contact with the toxic substance.
Procedures
A. Experimental Procedures
We will obtain the Spirogyra, Volvox, and Micrasteria, the types of algae we’ll use in the experiment. Each type of algae will be put into containers so we can record its growth. Each type of algae will be divided into 3 different tubes which differ in pH values 7.0,6.0, and 4.5, This means the normal algae cultivated in neutral water (7.0) , low acid water (6.0), and high-acid water (4.0). As time passes we’ll examine each alga with a microscope so that we can record any changes in cellular structure we see.
Data Analysis
Our hypothesis will be correct because like we said, we already know that the Spirogyra will have the most Hydrochloric Acid out of the three different types of algae because of it’s spiral structure. When we put the algae’s in the containers and put the Hydrochloric Acid, we have to keep checking up on the algae and how if it is progressing or not with the acid. We will keep this going for about 3 weeks and record the data every other day. We then will put all of the data in a graph that summarizes how the algae was cooperating with the acid and if that was good or bad.

Hazard levels: biological - There are a lot of Hazards when it comes to Hydrochloric Acid. It is a very corrosive acid, which means it’s capable of destroying solid