DNA replication is a biological process by which a molecule of DNA is copied. This process starts at the origin of replication where the double helix of the parental DNA is being unwound by the helicase in two separated strands. Then, single-strand biding proteins bind on the unpaired strands keeping them from repairing. The topoisomerase helps relieve the strain by breaking and swiveling the DNA strands ahead of the replication fork. The primase synthesize a short stretch of RNA called primer, which is the initial nucleotide chain synthesize during DNA replication. DNA-polymerase catalyzes the synthesis of new DNA by adding nucleotide to the preexisting chain of RNA. The double strands of the DNA is originated in opposite direction this why they form an antiparallel structure. The strand formed in same direction as the replication fork progress is called the leading strand and the one formed is the opposition direction of the fork is called the lagging strand. We have to notice that the lagging strand is synthesized discontinuously as a series of segment called Okazaki fragments. These fragments are joined together by the DNA ligase to form a continuous DNA strand.
B- RNA transcription
Transcription is the synthesis of RNA using the information in the DNA. The stretch of DNA that is transcribed into an RNA molecule is called the transcription unit. The transcription is divided into three stages:
1. Initiation, during this stage, RNA polymerase binds to the promoter located on DNA strands. In eukaryote, some proteins called transcription factors bind to the promoter and mediate the binding of RNA polymerase what forms the transcription initiation complex, which mark the beginning of
* Gregor Mendel had no experience with DNA – did pea pod experiment * Proteins at one point were considered as the genetic material * Miescher: found a phosphorus rich substance in puss cells * Levene: isolated 2 types of nucleic acids * Hammerling: worked with acetabularia. (Reciprocal graft experiment) * Griffith: wanted to make a vaccine. * Worked with Live S strain and R strain. * A chemical substance from one cell is capable of genetically transforming another…
about the exact process of how DNA is actually replicated, I remember learning about it briefly in high school, but I was never really interested. However, now that I actually understand all the processes leading up to DNA replication and genetics, it makes it much more interesting. Way back in 1953 Watson and Crick wrote two papers about how they thought DNA replication might work. They predicted that first hydrogen bonds connecting the two strands of the DNA molecule are broken. When this…
7.2 DNA Replication 7.2.1 State that DNA replication occurs in a 5' - 3' direction DNA replication is semi-conservative, meaning that a new strand is synthesised from an original template strand DNA replication occurs in a 5' - 3' direction, in that new nucleotides are added to the C3 hydroxyl group such that the strand grows from the 3' end This means that the DNA polymerase enzyme responsible for adding new nucleotides moves along the original template strand in a 3' - 5' direction Direction…
MODELING Replication Use two zipping plastic bags to model how complementary strands of DNA attach to template strands during replication. PROCEDURE MATERIALS • 2 zipping bags • scissors 1. Cut the sliding zippers off both bags. One zipper represents the template strands of a DNA molecule. 2. Cut the other zipper into four smaller pieces and unzip each of them. These represent free nucleotides. Don’t worry about which nucleotide is which in this activity. 3. Use the pieces to model replication as shown…
1.DNA molecules are made of two polymers connected by the hydrogen atom bonds and coiled in the shape of a double helix. The 3 parts are the deoxyribose(sugar), Phospate group 5 carbon base 2.DNA molecules are made of two polymers connected by the hydrogen atom bonds and coiled in the shape of a double helix. 3.A purine is a nitrogenous base which has two carbon-nitrogen rings (Adenine and guanine) whilst pyramidine only has one carbon-nitrogen ring (Thymine and cytosine). 4.1. Components of…
BSC: Exam 3 (chapters 16-20) Before lecture Questions: Q: “A discrete unit of hereditary information consisting of a specific nucleotide sequence in DNA (or RNA, in some viruses).” A: gene Q: If one strand of a DNA molecule has the sequence of bases 5'-GATTACA-3', the other complementary strand would have the sequence 3'-CTAAGTG-5' The two strands of the double helix are complementary, each the predictable counterpart of the other Adenine (A) always pairs with Thymine (T); and Guanine…
responsibility for posting refined answers to the guided readings - succinct, relevant, clear, and with pictures or a video to compliment. When contributing to the reading guide, follow these steps: 1) First complete the reading guide on your own from the DNA unit page. 2) Write your response to a question in word and then copy it. Be sure to upload pictures and/or video for each question. 3) Click on the edit button and then go to the appropriate question and paste your answer below it. Sign your contribution…
sugar that is a component of DNA nucleotides Nitrogenous base- an organic base that contains nitrogen, such as a purine or pyrimidine; a subunit of a nucleotide in DNA and RNA Purine- a nitrogenous base that has a dou- ble-ring structure; one of the two general categories of nitrogenous bases found in DNA and RNA; either adenine or guanine Pyrimidine- a nitrogenous base that has a single-ring structure; one of the two gen- eral categories of nitrogenous bases found in DNA and RNA; thymine, cyto- sine…
made up of proteins(60%) called histones and DNA(40%) the reacae was on to determine which of these organic molecules actually determined or controlled the heritable traits passed from generation to generation. finally concluded to be dna based on the hershey chase blender experiment (though mccarty macleod actually determined it first but the scientific community didn't believe it could be dna). next the race was on to determine the exact structure of dna which was determined by watson and crick in…
Where does DNA replication take place and how does DNA replication occur? Dear Auntie Lisa, Congratulations on your pregnancy! My mom just called and told me, I’m so happy for you and Erv! Now, I know you’re nervous about this pregnancy (being as how your past 30 years old, but a young 32 nonetheless!), and you asked my mom questions about how the baby actually becomes a baby, ie how it inherits genes from your and Erv’s DNA, where the actual “passing on” of genes occurs, but have no fear! Myself…