Histology
Laszlo Vass, Ed.D. Version 42-0013-00-01

Lab RepoRt assistant

This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable file which can be sent to an instructor.

Purpose

What is the purpose of this exercise? To look at cells through I microscope

Are there any safety concerns associated with this exercise? If so, list what they are and what precautions should be taken. Be careful with the microscope, make sure you hold it right.

Exercise 1: Epithelial Tissue

Data Table 1: Epithelial Tissue Observations
TISSUE TYPE OBSERVATIONS

Simple Squamous Many little circles, with large circles surrounded by horizontal long cells (purple)

Simple Cuboidal Long skinny cells, in no apparent order, with a larger odd shaped call with thick walls next to it. (purple)

Simple Columnar (stomach) One long straight mass of cells with smaller circular cells next to it with and uneven edge (purple)

Simple Columnar (duodenum) Column shaped cells not in a straight line with cube like cells below it (pinkish-purple)

Stratified Squamous (keratinized) Long mass of cells, horizontal, with oval shaped mass of cells next to it (purple)

Stratified Squamous (non-keratinized) A bunch of cells in little ovals

Pseudostratified Ciliated Columnar Thin line in a rounded shape with circular cells in the middle and longer thicker cells on the side (purple)

Transitional Almost paisley looking cells in a not straight organization (red/purple)

Stratified Cuboidal (online) Circles of cubed cells

Stratified Columnar (online) Column cells in a rounded shape with cube cells on one side and blank on the other

Questions

A. Why is the study of histology important in the overall understanding of anatomy and physiology? So you can actually see what the cells and tissues inside your own body look like and why they look like that in a functional way.

B. How are epithelial tissues named? They are named after what function they perform.

C. Why are some epithelial tissues stratified? They are the cells that are attached to the deepest layer of the basement membrane.

D. Unlike squamous cells, cuboidal and columnar cells have large, open cytoplasm. Which functions of epithelial tissue are supported by having such big cells? They need a lot of space to house the organelles required to process materials taken up into the cell.

E. Look at the following drawings and identify each type of epithelial tissue:

1. stratisfied squamous keratinized

2. simple columnar

3. pseudostratisfied columnar

4. stratisfied squamous nonkeritanized

Exercise 2: Connective Tissue obseRvations Data Table 2: Connective Tissue

Tissue Amount and Shape of
Cells Amount of
Matrix Are there fibers? If so, are they parallel or scattered? Mesenchyme (online) Many tadpole looking cells A lot scattered

Aerolar (online) A lot of cells ranging in shape Medium amount scattered
Adipose (online) Many circles next to each other Not much no

Dense Irregular (online) Long cells, many Very little parallel

Reticular Many circle cells in between lines Not much scattered

Dense Regular: Tendon Many in straight lines A good amount parallel

Hyaline Cartilage Not many circular cells surrounded by open space A lot no

Elastic Cartilage Many oval like cells Not much no

Fibrocartilage A good amount of long cells A lot parallel

Compact Bone Large cirles surrounded by smaller circles like a tree trunk A