Manipulating Enzymes Lab
Honors Biology, P.1
Anthea Munoz
Carmen Donate
Weston Gray
Dustin Morris Liver Enzymes Reaction Rate with Temperature 2. Hypothesis
There is a relationship between reaction rate of an enzyme (catalase) and temperature. The reaction rate will increase with increases in temperature until optimal temperature is reached.
The enzyme will become inactive at the temperature at which catalase denature 3. Experimental Design
We measured the reaction rate of catalase in the liver by looking at the presence of bubbles which is originated by the generation of oxygen gas.
1. We measured the weight of each liver
2. We labeled each Liver A­E
3. We took the temperature of all the livers
4. We put liver A and Liver B in the refrigerator at the same time.
5. After 5 mins we took out liver A
6. We measured the temperature
7. We put hydrogen peroxide on the liver; then, we timed how long bubbles were being produced (reaction time)
8. After 10 mins, we removed Liver B from the refrigerator
9. We repeated steps 6 and 7
10. We then put liver C in the microwave for 5 sec
11. We repeated steps 6 and 7

12. We then put liver D in the microwave for 10 secs
13. We repeated steps 6 and 7
14. We then took liver E
15. We repeated steps 6 and 7 4.Data
Liver A was green on top and red around, when we applied hydrogen peroxide (amount not measured) it started to fizz white foam (time not recorded) and then started to bubble for 42 sec.
The foam eventually started to turn a brown color. Liver B started to fizz for the first 6.33 sec then it started to bubble for the 27 sec.
Liver C was brown and green and when we applied the hydrogen peroxide it fizzed with little bubbles for 45 sec, the bubbles stayed unpopped for about a minute or two.
Liver D was red and when we applied hydrogen peroxide it started to fizz for 5.66 sec then bigger bubbles started to appear for the rest of the 18 secs.
Liver E fizzed immediately and made a foam mixture and then big bubbles appeared only on the left hand side of the liver for a total of 26 secs.

Temperature

Temperature

before

after

Reaction Time

Liver A

4.7

22˚C

11˚C

29 sec

Liver B

4.7

21˚C

18˚C

33 sec

Liver C

4.8

21˚C

42˚C

45 sec

Liver D

4.7

22˚C

65˚C

18 sec

Liver E

4.8

23˚C

23˚C

26 sec

Weight

5.

Conclusion ­We tested how altering the temperature of the liver affected the reaction rate of catalase. To change the temperature of the five livers, we refrigerated livers A and B and microwaved livers
C and D, leaving liver E at room temperature as our control group. To quantitatively measure changes of “reaction rate” with temperature, we noted the temperature of the liver after alteration and how long the reaction was. The heated livers yielded the best reaction