MANIPULATION OF BACTERIA INTRODUCTION: In this experiment that we performed, there were many methods that were used to help us manipulate and identify the bacteria E.coli on a MacConkey agar plate. The first part of the experiment involved the methods of manipulating, identifying and counting the bacteria and the second part was to find out whether the bacteria E.coli was the only type found in the given area by gram staining. E.coli was the chosen bacteria for this type of experiment. It is a gram negative bacterium that will grow rapidly given ‘any culture medium with the necessary energy source, nutrients, pH, and temperature’. Therefore, MacConkey Agar being the medium for its growth will enable us to achieve the experiment…show more content… You then spread out 100ul of the three dilutions onto the centre of different Agar plates using a loop and leave it to dry. This is to enable an evenly spread over the surface. After incubation for a few days, the colonies should be formed on each Agar plate ready to be examined, counted and recorded. Taking into account the dilution factor and the volume spread on each plate which is 100ul, the bacteria in 1ml of the original culture is calculated.
METHOD FOR GRAM STAINING Choose your selected colony ready to use on a clean slide so that no contamination is made. A drop of sterile saline is put onto the slide and the smear of the culture is mixed into it and spread it out thinly so that there is no overlap to enable clear image after staining. Then leave it to dry so that it doesn’t drip and mess the area or slide and pass it two or three times under the Bunsen burner for the hydration of the remaining liquid. Using the following gram staining method, stain the slide: -Start by squirting with the crystal violet solution and leaving for about 30 seconds then rinsing off with water -Then use Grams iodine solution and leaves for 30 seconds again and wash it off with water. -The decolouriser stage is the third stage where acetone/alcohol is squirted and left only for about 5-10 seconds this time and rinse
worldwide. India on the brink of famine due to overpopulation during the mid 20th century went from famine to self-sustaining cereal producers within 2 decades when Norman Borlaug’s High yielding wheat (HYW) variety was introduced. The genetic manipulation of bacteria, in particular E.Coli, using Recombinant DNA techniques has resulted in huge advances in medical treatment; the production of synthetic human proteins such as insulin as a supplement for patients that cannot synthesize their own proteins…
last 20 years, there has been great improvements in technology, in general, but more specifically in biotechnology. Biotechnology is ‘the use of living cells, bacteria, etc., to make useful products (such as crops that insects are less likely to destroy or new kinds of medicine).’(Merriam-Webster, 2014) So much is learned by the manipulation of organisms, for the greater good. Ways are known, now, how to treat various fatal diseases, such as cancers, while others, like Polio, there are vaccinations…
Structural Diversity: is the divisions in a community that result from having many different physical characteristics Protista: free-living or colonial organisms with diverse nutritional and reproductive modes, diverse group of eukaryotic microorganisms Bacteria: a member of a large group of unicellular microorganisms lacking organelles and an organized nucleus, including some that can cause disease, consist of prokaryotic microorganisms Fungi: an organism of the kingdom Fungi lacking chlorophyll and feeding…
Genetic Engineering: An In-depth Dissolution Genetic engineering, also referred to as genetic manipulation, is the process of modifying an organism’s DNA structure and/or sequence using biotechnology. Genetic material is altered using restriction enzymes and gel electrophoresis. These two methods are described by the removal, or splicing, of a specified part of the genetic code, and then introducing the altered code to a new DNA which may be from natural sources, or formulated in an artificial…
Pedigree – chart of the phenotypes and genotypes in a family that is used to determine whether an individual is a carrier of a recessive allele. Karyotype – image of all of the chromosome in a cell Chapter 8.1 Bacteriophage – virus that infects bacteria Chapter 8.2 Nucleotide – monomer that forms DNA and has a phosphate group, a sugar, and a nitrogen base Double helix – model that compares the structure of a DNA molecule, in which two strands wind around one another, to that of a twisted ladder…
Topic 1 2012 Basic Nucleic Acid Isolation, Manipulation and Analysis Key points:- DNA and RNA easily purified but as whole cellular populations, not specific sequences. RNA preps. can have significant DNA contamination. DNA in plasmids or phage can be purified selectively. Oligonucleotides can be synthesized chemically but efficiency and purity decrease with length. Also, product has 5’ –OH after de-protection. Variants can be made with unnatural bases or variations on phosphodiester…
Aseptic Non-Touch Technique Background; http://www.infectioncontrolmanual.co.ni/index.php?option=com_content&view=category&id=141&Itemid=121 Aseptic non-touch technique aims to prevent the introduction of micro-organisms into susceptible body sites. Aseptic non-touch technique endeavours to prevent the introduction of micro-organisms into susceptible body sites, as may occur during invasive procedures. Its aim is asepsis, i.e. “the absence of pathogenic organisms or their toxins from the blood…
Meningitis Tubercular Meningitis Rosenie Beausejour Med-Life Institute June 6th, 2013 Abstract Tubercular Meningitis is a rare but infectious disorder caused by a bacterium. The name of the bacteria is mycobacterium tuberculosis. What happens is that the bacterium infects the meninges, which is the membrane that covers the Central Nervous System. When this happens, the membrane around the spinal cord and the brain becomes inflamed. Some risk factors may include having a history or AIDS…
and the pumping of the nodes during Lymphatic massage stimulates the production of various white blood cells and thus boosts the immune system. It is ideal for assisting the body during a cleanse or fast, as the nodes are where cell debris, and bacteria are broken down and processed, and invading organisms and disease cells are neutralized. It is safe for most everyone to receive,…
and essential techniques in the study of DNA. The extraction of DNA from cells and its purification are of primary importance to the field of biotechnology and forensics. Extraction and purification of DNA are the first steps in the analysis and manipulation of DNA that allow scientists to detect genetic disorders, produce DNA fingerprints of individuals, and even create genetically engineered organisms that can produce beneficial products such as insulin, antibiotics, and hormones. (www.biologyjunction…
