***Don’t forget that on 03 Oct, he transitioned to “information covered on Exam 3” starting with “How to construct a plasmid” http://quizlet.com/54570964/flashcards Steps to construct a plasmid 1. Amplify the gene of interest with PCR 2. Use Restriction Digest Enzymes to cut the gene and insert into plasmid 3. Insert plasmid into bacteria of interest 4. By using chemical transformation, electroporation (electroshock), conjugation, or bacteriophage 5. Grow bacteria on selective media…
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