cellular machinery. Once excised the insertion region must include TA sites at each end for successful transposition. However the transposase may also be designed to be encoded on the same plasmid as the transgene, or introduced in vitro directly into the cell in the form of coding mRNA. Insertion of the transposon is largely considered to be random since it only requires a TA site at the target sequence for integration. However a study has shown half the transpositions have preference for 10% of the total…
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