DNA Extraction: Genomic DNA is found in the nucleus of eukaryotic cells. Mitochondria contain singlestranded DNA from our mother. Reagents Used in Lab: ● Lysis Buffer: Disolves the cells’ nuclear membranes and the phospholipid bilayer, allowing for the DNA to be released ● Protease: Breaks down cellular proteins. We used protease to DESTROY DNAses and the histones. ● Cold Alcohol: to precipitate the DNA out of the solution. The DNA is less soluble in alcohol, so it WILL become visible…
Bacterial Identification Lab The purpose of the lab is to familiarize you with the science and techniques used to identify different types of bacteria based on their DNA sequence. Not long ago, DNA sequencing was a time-consuming, tedious process. With readily available commercial equipment and kits, it is now routine. The techniques used in this lab are applicable in a wide variety of settings, including scientific research and forensic labs. Basic Steps • Prepare a sample from a patient and…
scientific personnel needed to tackle the job. Processing the current backlog of forensic DNA evidence and expanding local, state, and national databases of DNA evidence obtained from crime scenes, as well as from convicted offenders, will require a massive effort. THE WORK is quite literally piling up. In STARTING POINT Myriad Genetics lab technician Drew Carter opens evidence bags containing individual DNA samples collected by New York State Police from the site of the World Trade Center disaster…
Pre IB Biology Lab #3: DNA Extraction Prepared For: Courtney Oliver By: Adefemi Obisanya Date: December 21, 2012 In this lab, the researcher purpose for conducting this experiment is to become familiar with a procedure for extracting DNA, collect DNA samples and observe the physical characteristics of DNA. The hypothesis is that the DNA extracted from the strawberry will look like clump strands that cannot be seen by the naked eye. Materials Zip…
cellular structure and composition as well as the functions of individual structures in the cell as they relate to the organism as a whole. This course will also include information on genetics as it relates to heredity as well as discussions on how DNA contains all of the information for creating a living organism. We will also be discussing Biology on a larger scale with topics such as ecology and evolution. There will also be a variety of laboratory exercises and projects which are designed to…
Transformation Lab Report I. Pre-lab Bacterial transformation occurs when an organism receives DNA from a different species and expresses the implemented gene, thus becoming a transgenic organism. The practice proceeds as follows: first cut the plasmid from one organism and DNA from another, using the same restriction enzyme on a palindromic sequence. The recombinant plasmids then enter the host bacteria and asexually reproduce infinitely. We can identify that the bacteria has the new DNA by testing…
separate and view macromolecules (large molecules, such as DNA, RNA and proteins). We used this method to separate each suspects DNA into fragments using enzymes to break apart the DNA so that it can be broken down into smaller ring pieces. We poured the DNA found at the crime scene and the DNA of the suspects on the minus pole of the Gel. After a day, the results were ready. We determined who the murderer was in this final stage by counting the DNA rings of each suspect trying to figure out which group…
******************************************************************************************** Answer Sheet—Module 7 Lab DNA Extraction Click on the following link and view the DNA extraction: http://learn.genetics.utah.edu/content/labs/extraction/ 1. What is the source of the cells used in this demonstration? A human. 2. Give three practical uses of DNA that is extracted: a. Genetic testing b. Body Identification c. Forensic analysis 3. Name the piece of equipment…
Lab Exercise DNA Extraction 10/30/13 DNA in all organisms is found primarily in chromosomes. Eukaryotic cells, like those found in humans, contain several linear chromosomes. Prokaryotic cells, such as bacteria, contain one large circular chromosome. DNA is composed of building blocks called nucleotides, each made up of a five-carbon sugar (deoxyribose), a phosphate group, and a nitrogenous base. The four kinds of nucleotides differ only in their nitrogenous bases. It is…
Transformation Lab Report Introduction Transformation is the transfers of virulence from one cell to another, through the transferring of genetic material. It was originally postulated in 1928 through the works of Federick Griffith, a British microbiologist. Griffith observed that the mutant form, non-virulent form, of the bacteria Streptococcus Pnumoniae could be transformed into the normal, virulent form, when injected into mice along with heat killed normal forms. He concluded that somehow…