see larger image In 1966, B. Weiss and C.C. Richardson isolated DNA ligase, an enzyme that could be used to “ligate” or paste together two strands of DNA. Thus, researchers could now attach two or more DNA molecules or break it into fragments. In 1972, Paul Berg used restriction enzyme to cut DNA, and then a ligase to paste two DNA strands together to form a hybrid circular molecule -- this was the first “recombinant” DNA. This ushered in a new era of genetic manipulation -- what has come to…
Words 1179 - Pages 5